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Philpatrick

Squamellaria pollen

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Widefield fluorescence micrograph of hydrated Squamellaria pollen.

The pollen above was collected from a plant identified as Squamellaria. It has been stained to distinguish features. The linear aperture, the colpus (colpi plural), emits a yellow color on the pollen circumference. The red color is the exine.

 

 

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Dry Squamellaria pollen imaged with reflected light.Unnamed_190322_112813-2.thumb.jpg.3a2ff4a4cfdecac5e1e36b4571da2772.jpg

In the autofluorescence image above the pollen is fresh and has not been stained. The pollen appears to be tricolpate, meaning there are three colpi. The cross section of the pollen in the polar orientation shows the three colpi simultaneously (blue arrows). The colpi are the three furrows in the circumference of the pollen wall.

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Stained pollen surface detail.

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I am working on more detailed images.

 

 

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I had to recheck, restain and reimage a pollen sample to find that the pollen features were consistent. In the last image I think it is interesting because this pollen looks like the exine has a croton pattern, you can see the raised croton pattern especially in the last image from the set above. It looks like interconnected rings with 5/6 triangular columns on the muri. The raised areas on the exine appear darker in color. The muri is the raised area forming the reticulated pattern, the columns on the muri form the croton pattern. The lumina are the spaces between the muri. The brochus, is a lumen ( singular of lumina ) including half the width of the muri. To measure a brochus ( singular of brochi ), measure a lumen including half the width of the muri; halfway into the muri surrounding the lumen. The measurement of that space, the brochus, could be useful to know.

The pollen has an exine pattern that closely resembles a croton pattern, but it could be another type of exine patterning. More resolved images will help identify this.

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Hydrated, stained, transmitted light brightfield.

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